Enhanced FTIR bench-top imaging of single biological cells.

FTIR spectroscopic imaging of live cancer cells

FTIR spectroscopic imaging allows the visualization of the distribution of chemical components in cells. However, obtaining images of living cells is difficult because of the strong absorption of water in the mid-infrared region. The use of attenuated total reflection (ATR) FTIR spectroscopic imaging provides a possibility to study tissues or live cells in an aqueous environment.[1] This approa...

Effect of Peptide Derived from Scorpion Toxin on Enhanced Permeability of Doxorubicin Conjugated Gold Nanoparticles in HeLa and MDA-MB-231 Cells

Background: Cell penetrating peptides (CPPs) can enter a cell through the cell membrane, and used in the fields of drug delivery, gene therapy, and cancer therapy by their property transporting various molecules into cytoplasm. Gold nanospheres (GNSs) are a useful tool for molecular imaging, because they are not cytotoxic and have high solubility, excellent light scattering property and ease of...

High-resolution FTIR imaging of colon tissues for elucidation of individual cellular and histopathological features.

Novel technologies that could complement current histopathology based cancer diagnostic methods are under examination. In this endeavour mid-infrared spectroscopic imaging is a promising candidate that can provide valuable bio-molecular information from unstained cells and tissues in a rapid and a non-destructive manner. With this imaging technique, the biochemical information obtained from sma...

A Comparison of Fundamental Performance between Two Bench Top Clinical Chemistry Analyzers

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The label-free, non-destructive chemical analysis offered by FTIR spectroscopic imaging is a very attractive and potentially powerful tool for studies of live biological cells. FTIR imaging of live cells is a challenging task, due to the fact that cells are cultured in an aqueous environment. While the synchrotron facility has proven to be a valuable tool for FTIR microspectroscopic studies of ...